Glut1 flow antibody
WebTranslocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking. Fig. 1. Western blot analysis of extracts of various cell lines, using GLUT4 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG (H+L) (A0208) at 1:1000 dilution. WebMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the C terminal of Glut1 protein. Background Glucose transporter 1 (Glut1, SLC2A1) is a widely expressed transport protein that displays a broad range of substrate specificity in transporting a number of different aldose sugars as well as an oxidized ...
Glut1 flow antibody
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WebConfocal immunofluorescent analysis of fixed frozen mouse hippocampus labeled with Glut1 (E4S6I) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP ® Isotype … WebThe cells were then stained with either Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human GLUT1 antibody (Cat. No. 569293; solid line histogram) at 0.06 µg/test. The fluorescence histogram showing the expression of GLUT1 (or Ig Isotype control staining) was derived ...
WebGlucose transporter 1 ( GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols …
WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. PE Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1. Suitable for: … WebCited in 1 publications. View Rabbit Polyclonal anti-Glut1 Antibody [Alexa Fluor® 488] (NB110-39113AF488). Validated Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P. Validated Species: Human, Mouse, Rat + more.Range of directly conjugated antibodies available.
WebAlthough Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post …
WebIn WB a band is seen at ~55 kDa on kidney membrane preps representing GLUT1 protein. Depending on the tissue and any post-translational modifications, this protein can run anywhere between 40-60 kDa. This … heart flutters all the timeWebFlow cytometric analysis of human GLUT1 expression on human HEP G2 cells. Cells from the human Hep G2 (Hepatocellular carcinoma, ATCC HB-8065) cell line were stained … mounted cell phoneWebAnti-GLUT1 antibodies are available for the flow cytometric detection of the target protein, 'solute carrier family 2 member 1'. In humans, this target is encoded by the GLUT1 gene, … heart flutters and lightheadednessWebNotes. 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75784). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. mounted cerberusWebJan 26, 2024 · Glut1 Antibody (A-4) is an IgG 1 κ mouse monoclonal Glut1 antibody (also designated Glucose Transporter GLUT1 antibody, SLC2A1 antibody or HepG2 glucose transporter antibody) suitable for the detection of the Glut1 protein of human origin by WB, IP, IF, IHC(P) and ELISA. Glut1 Antibody (A-4) is available as both the non-conjugated … mounted centarWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. heart flutters and headacheWebMar 30, 2024 · Flow cytometry. For flow cytometry, cells were seeded 24 h before analysis and then detached from the well surface using a short StemPro Accutase treatment (Thermo Scientific). Approximately 0.5 × 10 6 cells were harvested per sample. Samples were washed and subsequently incubated with 5 μL GLUT1 binding domain (H2-RBD-GFP, … heart flutters and dizziness women