Cytoplasmic asters

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WebJan 1, 2005 · The cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a spindle-like ... WebUnlike the oocytes of most other animal species, unfertilized murine oocytes contain cytoplasmic asters, which act as microtubule-organizing centers following fertilization. This study examined the role of asters during the first cell cycle of mouse nuclear transfer (NT) embryos. NT was performed by intracytoplasmic injection of cumulus cells. Cytoplasmic …

Cortical cytasters: a highly conserved developmental trait of Bilateri…

WebFeb 16, 1999 · Thus, the sperm centrosome must have recruited γ-tubulin from the maternal cytoplasm and gained the ability to nucleate Mts and form asters after entry into the oocyte. Surprisingly, 20 min after fertilization, during metaphase of meiosis I, neither sperm asters nor γ-tubulin staining was found associated with sperm nuclei (Fig. 1 C ). WebMar 15, 2012 · In the next experiment, multiple asters observed in 5.5 vs 8 hpi pronuclear zygotes were located near the male pronucleus, suggesting that those multiple asters were not the cytoplasmic asters of maternal origin. In conclusion, multiple aster formation frequently observed in vitrified-warmed bovine oocytes may be related to loss of … cylinder records value

MEK1/2 is a critical regulator of microtubule assembly and

WebJan 1, 2005 · Cytoplasmic asters were localized by staining with an anti-alpha-tubulin antibody. Enucleation of MII oocytes caused no significant change in the number of … WebSep 1, 1998 · These unique results not only provide a demonstration that cytoplasmic dynein is required for the formation of mitotic asters in N. haematococca; they also represent the first report of mitotic phenotypes in a dynein mutant of any filamentous fungus and the first cytoplasmic dynein mutant of any organism whose mitotic phenotypes … WebThe cytoplasmic foci were larger and more abundant than in untreated oocytes (Table 1). Double staining revealed that the anti-γ- tubulin reactivity was found in the center of all the asters... cylinder recordings

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High incidence of multiple aster formation in vitrified-warmed …

WebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a … WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation … cylinder record playerWebDec 7, 2024 · We observed all cytoplasmic networks mostly moved together inside asters. The highest velocity differences between networks occurred at the aster periphery. Mechanical integration between all … cylinder recovery vessel

"WebBackground: Cytasters (cytoplasmic asters) are centriole-based nucleation centers of microtubule polymerization that are observable in large numbers in the cortical … " - Cytoplasmic asters

Cytoplasmic asters

Fertilization in F. fimetaria eggs ( green microtubules, red DNA). a ...

WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation presumably involves the overriding of intrinsic control points that prevent centrosome self-assembly in dioic eggs, thus avoiding attempt to develop in the absence of a male gamete. Webnumerous astral microtubules (cytoplasmic asters) in the mature M2 oocyte cytoplasm. An astral microtubule aids in the male and female genome union during fertilization. In mouse oocytes, several pericentriolar material foci have been found in acentriolar astral microtubules (cytoplasmic asters) [10]. It is thought that the MTOCs of cytoplasmic ...

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Webarray of MTs organizes in clear and visible compartments that we call yolk cytoplasmic layer asters (YCL asters) (Fig. 1C–G), with MTs radially orienting from each of the domain’s center. To ... WebJan 1, 2012 · At the time of nuclear envelope breakdown (NEBD), the asters on the pronuclear surface massively polymerise microtubules toward the chromosomes due to Ran GTPase, whereas the cytoplasmic asters become destabilised and lose their polymerised microtubule signal, pointing toward an inactivation of their MTOC function.

WebDownload scientific diagram Fertilization in F. fimetaria eggs ( green microtubules, red DNA). a The sperm aster ( arrow ) is visible even in the oocyte cytoplasm during late metaphase ( large ... WebIn a number of studies Taxol has been reported to induce cytoplasmic MTs asters in cells treated with the drug [e.g., [9,10,28, 29]]. Most of these studies have used Taxol concentrations above 0. ...

WebNov 14, 2000 · Numerous cytoplasmic asters appeared at the cortex of both fertilized (not shown) and unfertilized eggs at the end of meiosis II (compare (i,j), which show meiosis II and the end of meiosis, respectively). (m–p) In unfertilized eggs, the asters migrated towards the female pronucleus and two associated with it (arrowheads in (o)) while the … WebFeb 1, 2005 · Cytoplasmic asters did not duplicate and were clearly seen at the surface of the parthenogenetic embryo until third and fourth mitoses (Figs. 8C, D). Download : …

WebFeb 22, 2024 · Asters are radial microtubule arrays found in animal cells. These star-shaped structures form around each pair of centrioles …

WebJan 23, 2012 · Of the 233 embryos belonging to the syncytial blastoderm mitoses, 61% (n = 142) progressed normally through the nuclear division cycles, and 39% (n = 91) showed a wide range of mitotic defects. Defects in chromosome … cylinder recordings were invented byWebThe cell cycle culminates in the division of the cytoplasm by cytokinesis. In a typical cell, cytokinesis accompanies every mitosis, although some cells, such as Drosophila embryos (discussed later) and vertebrate … cylinder regulator hsn codeWebApr 15, 2024 · The sperm aster is situated in the inner cytoplasm and its MTs are connected to the parallel MTs in the shear zone (Houliston and Elinson, 1991a). The cortex region consists of a plasma membrane, actin cytoskeletal filaments, and the endoplasmic reticulum (ER) ( Houliston and Elinson, 1991a ). cylinder reed switch mounting bracketWebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the … cylinder reed switch sensorWebFeb 15, 2024 · The cytoplasm is a complex material, whose rheological properties and their impact on cellular functions are emerging as important concepts in cell biology. Here, we probed cytoplasm … cylinder refill onlineWebJan 1, 2012 · The cores of these asters are composed of a few PCM components, including pericentrin and gamma-tubulin. Based on this composition and the ability of these … cylinder recycling toolWebMost insects have a single NP gene and multiple NPs and NP-like peptides are produced by extensive alternative splicing 3, 5 or the different processing of the precursor peptide. 2 Alternatively, various decapods 4 and several hemipteran insects 6, … cylinder refill price